- January 30, 2012
Identifying structural variation - January 25, 2012
Autism Speaks - RFA release
- November 29, 2011
9th Annual Affymetrix User Group Meeting -
December 8, 2011
Introducing the NEW Database of Genomic Variants!
NEW! Population Control DNA Samples Now Available
Healthy control DNA samples from the Ontario Population Genomics Platform are now available at a charge of $650 per 96-well plate (2 micrograms per sample). Custom
amounts can be prepared upon request. Please contact the facility for details.
The Cytogenomics and Genome Resources Facility provides a comprehensive suite of cytogenetic and physical mapping services, DNA samples, clones and other resources to researchers both in the academic community and in the private sector.
Please see our acknowledgment policy.
- Cytogenomics Overview
- Cytogenomics Pricing
- Sample Submission
- Genome Resources Overview
- Detailed Services and Resources
- Genome Resources Pricing
- Facility Contacts
The Cytogenomics Facility offers a wide variety of chromosomal analysis techniques for many different research applications. For certified hospital clinical and prenatal cytogenetics, please contact The Hospital for Sick Children's Cytogenetics Laboratory.
Services offered include:
- Karyotyping
- human, rodent, primate, cell lines and others
- mouse splenocytes
- mouse embryonic stem cells
- spectral karyotyping (SKY)
- FISH mapping (metaphase, interphase)
- Transgene insertion point mapping (G-to-FISH)
- Physical mapping
- ES/transgenic integration site mapping, copy number determination
- Breakpoint mapping (translocation, inversion, duplication)
- Fluorescent labeling of BAC clones (for FISH mapping by customers), in conjunction with the Genome Resources Facility
- Whole genome array Comparative Genomic Hybridization. This service is offered by the Microarray Analysis and Gene Expression Facility
Please complete a request form. Karyotype analysis includes a complete summary of results listing the number of cells counted and karyotyped, the chromosome modal number and the number of atypical non-clonal cells. For a sample report, click here.
Please note: Prices for cytogenomic and genome resource services were increased effective May 1st, 2010.
| Service | blood | lymphoblast | spleen | fibroblast | fibroblast - no culture |
ESC | IPS |
|---|---|---|---|---|---|---|---|
| Karyotyping | $500 | $500 | $625 | $750 | $600 | $600 | $600 |
| 2 colour interphase FISH | $550 | $550 | $675 | $700 | $575 | $575 | $575 |
| 3 colour interphase FISH | $625 | $625 | $750 | $775 | $650 | $650 | $650 |
| 4 colour interphase FISH | $700 | $700 | $825 | $850 | $725 | $725 | $725 |
| 1 colour metaphase FISH | $450 | $450 | $575 | $600 | $475 | $475 | $475 |
| 2 colour metaphase FISH | $525 | $525 | $650 | $675 | $550 | $550 | $550 |
| 3 colour metaphase FISH | $600 | $600 | $750 | $775 | $625 | $625 | $625 |
| 4 colour metaphase | $675 | $675 | $825 | $850 | $700 | $700 | $700 |
| Sequential G to FISH | $800 | $800 | $925 | $950 | $825 | $825 | $825 |
| Sequential G to SKY | $1,400 | $1,400 | $1,525 | $1,550 | $1,425 | $1,425 | $1,425 |
| Sequential G to FISH to SKY | $1,700 | $1,700 | $1,925 | $1,950 | $1,825 | $1,825 | $1,825 |
| SKY | $825 | $825 | $950 | $975 | $850 | $850 | $850 |
| $75Genomic clone retrieval & fluorescent labeling biotin or DIG, Spectrum orange or Spectrum green | |||||||
Submit samples using the LabLink server
For instructions on creating an account, placing an order etc., download the following documents.
- Blood culture and karyotyping:
2-5mL peripheral blood in Sodium Heparin collection tubes (green top) stored and transported at room temperature. - Lymphoblastoid line establishment for FISH experiments:
Children: 3-5mL peripheral blood in ACD collection tubes (yellow top)
Adults: 5 -8mL peripheral blood in ACD collection tubes (yellow top)
Blood samples to be stored and transported at room temperature. - Mouse splenocyte culture and karyotyping/ FISH studies:
Please contact the facility, giving some details of experimental requirement. - Mouse embryonic stem cell karyotyping/ FISH studies:
Please contact the facility giving some details of experimental requirement. - For Spectral karyotyping (SKY), karyotyping of other organisms or cell types, or other services not listed above, please contact the facility.
Sample Submission forms (Microsoft Excel format)
- Cytogenomics
- Fish probes, clone retrieval and DNA Exraction
- Genome Resources Library Screening
- OPGP control DNA
The Genome Resources Facility provides genomic mapping services including retrieval of clones from the on-site repository, genomic library screening, tissue expression profiling, database analysis, annotation and probe selection, and whole genome amplification (WGA). Specific highlights are as follows. Please note that there are restrictions on distribution of some of the sample and library resources. In these cases, experiments can be performed in-house at TCAG if necessary. For further information, please ask us.
Request services using the LabLink server. For instructions on creating an account, placing an order etc., download the following documents.
- Human Control DNA samples
- Genomic library screening
- Human BAC and P1, mouse BAC and P1, porcine BAC, canine BAC, human cosmid
- Clone Repository and individual clone retrieval
- Human BAC, YAC and P1, mouse BAC, porcine BAC, canine BAC, cosmids
- Human and mouse Mammalian Gene Collection (MGC) cDNA libraries
- Whole Genome Amplification (WGA)
- generates large amounts of genome-derived DNA for microsatellite analysis, SNP detection, comparative genomic hybridization (CGH) or other applications
- Database queries, annotation, probe selection and fluorescent labeling
- Multiple tissue expression profiling (mouse, human)
- Radiation and somatic cell hybrid mapping (mouse, human)
Please note that there are restrictions on distribution of some of these sample and library resources. For further information, please ask us.
| Human cDNA Library | Mouse cDNA Library |
| Research Genetics sequence - verified (IMAGE) collection | NIA/NIH 15,000 collection |
| Riken cDNA collection | |
| Mammalian Gene Collection (MGC) | Mammalian Gene Collection (MGC) |
| Please contact us if you require assistance obtaining other cDNA clones | |
*these samples are available for purchase but must be used in-house at TCAG. Additional control sets are available for purchase and shipping. Please contact us for details.
How do I know if TCAG has the full-length cDNA clone I am looking for?
- Find the IMAGE id or Genbank accession number of the clone.
- Go to http://image.hudsonalpha.org/IQ/bin/buildIQ?categoryChoice=clone%7EClone .
- Add the IMAGE number (number only, not the word 'IMAGE') to the 'Clone ID' input box
or
or add the Genbank accession number (use uppercase letters) to the 'Genbank Accession Number' input box- Click on the 'Check all' and 'Submit' buttons. Click on 'OK' when the submitted search dialog box appears.
- Note the collection name. TCAG currently carries:
IRAT plates 1-89, 93-137
IRAU plates 1-113
IRAV plates 1-84, 87-136, 139-152, 155-164
| Human cDNA Screening by PCR | Mouse cDNA Screening by PCR | |||||||||||||||||||||||||||
|
heart spleen brain testis lung liver |
| Human Genomic Control DNA Samples |
| OPGP Control DNA Samples |
| HGDP CEPH Diversity Panel |
| HapMap samples |
| Coriell Human Variation Caucasian Panel (HVCP) |
| Service | Cost |
|
Library screening and confirmation i. Genomic library screening (first probe) ii. Each additional probe iii.Clones retrieved based on library screening |
$300 $75 $20 |
| Clones ordered based on database identifier (typically, bacterial stab or equivalent) | $20 |
| Purified DNA from clone | $45 |
| Library replication | Please enquire |
We can accommodate a wide variety of requests for resources and experimental procedures not listed above. For information on pricing for services not listed above, please contact the facility.
The Centre for Applied Genomics
The Hospital for Sick Children
101 College Street (TMDT Building, East Tower)
Room - 14 602, Toronto ON M5G 1L7
Tel: (416) 813-7654 Ext. 3573 Email: